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measured as mass gains clearly indicated that the amide exchange within the central region of the peptide is extremely slow as compared to the terminal (solvent-exposed) segments (compare mass increases of the a10 + and a18 + in Figure 11.14). A detailed analysis of the local exchange patterns showed some (but rather slow) deuterium incorporation at the ends of the transmembrane segment (106), which can be attributed to limited hydrogen scrambling within the peptide ions prior to their fragmentation (108). 5 contains a more detailed discussion of issues related to internal proton mobility and hydrogen scrambling within gas phase protein and peptide ions. Alternatively, local dynamic fraying can be invoked to explain such behavior. Indeed, lipid bilayers are known to be dynamic systems and allow signi cant water penetration into the hydrophobic bulk (109). Hydrogen exchange in the transmembrane segments due to such penetration would be slow for several reasons. The penetration events are rather rare (109) and the hydrophobic environment may impose severe steric constraints on the water molecule. Furthermore, the dielectric constant in the membrane interior is much lower than that of water itself, which would also adversely affect the exchange rate. barcode generator excel kostenlos Barcodes in Excel 2016, Excel 2013 and Excel 365 - ActiveBarcode
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